The Neurobiology Imaging Facility provides following tissue clearing services. Please check with us if you are unsure which clearing protocol would work best for your samples.
The mouse brain clearing X-Clarity protocol can be used to clear many other tissue samples, e.g. heart, kidney, liver, spinal cord, and embryos, with minimal/no modification. Only the electrophoresis time/condition would be different, and that can be easily optimized.
The bone clearing X-Clarity protocol has an additional sample preparation step, i.e. de-calcification. There is no other major difference between the brain clearing protocol and the bone clearing protocol.
Staining and embedding